polyclonal goat anti mouse il 1ra  (R&D Systems)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The epistatic interrelationships of IL-1, IL-1 receptor antagonist, and the type I IL-1 receptor.

doi: 10.4049/jimmunol.169.1.393

Figure Lengend Snippet: FIGURE 1. Murine protein isoforms of IL-1ra. A, Schematic diagram showing the relationships between the murine IL-1ra protein isoforms. Shared sequences are in black, those unique to pro-sIL-1ra (namely the signal peptide) are in gray, those unique to icIL-1ra1 (alternate exon 1) are in white, and the N-terminus that is deleted from icIL-1ra3 is hatched. B and C, Detection of the various proteins isoforms by Western blot of liver proteins from mice challenged with LPS (B) and skin proteins from un- challenged mice (C) of wt, rako, ratg, and rako, ratg mutants. The different isoforms of IL-1ra were assigned based on size relative to m.w. markers and recombinant sIL-1ra (R&D Systems), partial sequencing, and tissue distribution.

Article Snippet: Membranes were blocked for 1–2 h at room temperature in 10% nonfat dry milk in PBS/Tween, washed, incubated with 0.2 g/ml biotinylated polyclonal goat anti-mouse IL-1ra Ab (R&D Systems) for 1 h at room temperature, washed again, and finally incubated with streptavidin-HRP (1/1,000 dilution; Amersham Pharmacia Biotech, Piscataway, NJ).

Techniques: Western Blot, Recombinant, Sequencing